Regulatory

Part:BBa_K3024010:Design

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-09-28)


Double Promoter (Induced by arabinose, repressed by TetR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_I739101 is derived from BBa_J23100 and BBa_R0040. However we found that the double operator sequence of BBa_I739101 is different from BBa_R0040. We chose to use the sequence that was used in BBa_I739101.

Source

Sequence taken from BBa_K206000 and BBa_I739101

References